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KMID : 0382619850050020379
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Hanyang Journal of Medicine
1985 Volume.5 No. 2 p.379 ~ p.389
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An Electron Microscopic Study on the Organelles of the Hepatocytes in Chlorobenzilate Treated Albino Mice
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安基亮/Ahn, Ki-Ryang
김완식/鄭鎬三/李圭植/Kim, Wan-Sek/Chung, Ho-Sam/Lee, Chung Kyu
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Abstract
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Chlorobenzilate which is the organophosphorus acaricide, is very toxic to man and livestock. Chlorobenzilate is accumulated in the fat tissue like other organo-phosphorus insecticide so that it is extremely toxic. Its toxicity on the central ner-vous system produce extreme convulsion but its mechanism has not been under-stood. And also the degenerative changes in the liver, kidney and brain are appeared by its chronic toxicity.
Chlorobenzilate is hydrolysed in the liver that its metabolites may damage the hepatic parenchymal cells. So the authors have undertaken to pursue the toxic effect of the chlorobenzilate on the organelles of the liver cells.
Albino mice were used as experimental animals. The experimental animals sacrificed at 6 and 24 hours after administration of 641 mg (0.5m1) of chlorobenzilate per Kilogram of body weight. The specimens obtained from the liver were fixed in 2% glutaraldehyde-2.5% paraformaldehyde phosphate buffer (pH 7.2) and sliced with 600-800 A° thickness.
The specimens were stained with uranyl acetate and lead nitrate and then observ-ed with the electron microscope.
The results were as follows.
1. Proliferation of smooth endoplasmic reticulum and depletion of glycogen par-ticles were observed.
2. An increase in the number of secondary lysosomes, multivesicular bodies, and lysosomes were observed.
3. The atropy of the cisternae of the Golgi complex was observed. The disrup-tion of the cisternae of rough endoplasmic reticulum was associated with detachment of the membrane bound ribosomes.
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